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KMID : 0359320130530040211
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Korean Journal of Veterinary Research
2013 Volume.53 No. 4 p.211 ~ p.216
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Development and evaluation of protective capacity of Salmonella Enteritidis polyphosphate kinase-deleted and temperature-sensitive mutant
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Kim Ki-Ju
Park So-Yeon
Cho Young-Jae
Kwak Jeong-Yeon
Kang Zheng-Wu
Kim Eun-Hee
Choi Hwan-Won
Won Ho-Keun
Noh Yun-Hee
Hahn Tae-Wook
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Abstract
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This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed crossprotection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.
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KEYWORD
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cross protection, polyphosphate kinase deleted mutant, Salmonella Enteritidis, temperature-sensitive mutant
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